Journal: Bioactive Materials

Article Title: Osteoporosis treatment via programmed delivery of IRF5 siRNA to macrophages in bone tissue combined with ROS scavenging

doi: 10.1016/j.bioactmat.2026.10.015

Figure Lengend Snippet: siP@WM normalizes the high bone turnover state in ovariectomy-induced osteoporotic mice. (a) Confocal fluorescence images showing nanoparticle-associated fluorescence (red dots) in undecalcified bone sections after 4 weeks of treatment; (b) Osteoclast activity in femora after treatment, shown as (i) representative tartrate-resistant acid phosphatase (TRAP) staining images of decalcified femur sections and (ii) quantification of TRAP + cell number per mm 2 ; (c) Serum levels of bone turnover markers, including C-terminal telopeptide of type I collagen (CTX-Ⅰ) and procollagen type I N-terminal propeptide (P1NP), measured by ELISA; (d) Dynamic histomorphometric analysis of bone formation by sequential fluorescent labeling, shown as (i) quantification of mineral apposition and (ii) representative fluorescence images of femur sections. Green: calcein; yellow: tetracycline. Data are presented as mean ± SD (n = 3 mice per group). Statistical significance was determined by one-way ANOVA followed by Tukey's multiple-comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; ns, not significant.

Article Snippet: In order to assess potential effects of nanoparticles on bone metabolism, serum levels of mouse bone turnover markers, including procollagen type I N-terminal propeptide (P1NP, Cusabio) and C-terminal telopeptide of type I collagen (CTX-I, Elabscience), were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturers’ protocols.

Techniques: Confocal, Fluorescence, Activity Assay, Staining, Enzyme-linked Immunosorbent Assay, Analysis, Labeling

Journal: Cardiovascular Therapeutics

Article Title: CircIdh2 Attenuates Endothelial‐to‐Mesenchymal Transition and Myocardial Fibrosis and Exhibits Translational Potential

doi: 10.1155/cdr/2005876

Figure Lengend Snippet: AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies against Collagen I (1:200, #66761‐1‐Ig, Proteintech) and TGF‐ β 1 (1:200, #81746‐2‐RR, Proteintech).

Techniques: Marker, In Vivo, H&E Stain, Staining, Western Blot, Analysis